The review analysed five studies, revealing that infrasound exposure in animal models improved fracture healing, bone mineral density, and expression of key markers such as CGRP and survivin. These effects may be mediated by neurogenic and osteogenic pathways.  

Audible low-frequency sound, particularly around 100 Hz, was shown to increase fibroblast migration and metabolic activity in vitro. Directional application of sound waves influenced outcomes, with horizontal stimulation improving cell motility and vertical application producing inhibitory effects. In murine models, 20 kHz sound accelerated recovery of the skin barrier by enhancing keratinocyte activity and lamellar body secretion.  

While results are promising, the review emphasises limitations in sample size, heterogeneity of methods, and lack of clinical data. Standardisation of acoustic parameters and further mechanistic studies are necessary before clinical translation.  

The findings support the potential of non-invasive sound-based therapies as adjuncts in wound healing and bone regeneration, particularly for chronic or hard-to-heal injuries.